A synthetic peptide from rat c Fos protein conjugated to blue carrier protein has been used as the antigen. The peptide is homologous in mouse and human.
Proto-oncogene protein cFOS, c-Fos
FUNCTION: Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation.
SUBUNIT: Heterodimer. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA. Interacts with MAFB
SUBCELLULAR LOCATION: Nucleus.
INDUCTION: C-fos expression increases upon a variety of stimuli, including growth factors, cytokines, neurotransmitters, polypeptide hormones, stress and cell injury.
SIMILARITY: Belongs to the bZIP family. Fos subfamily
SIMILARITY: Contains 1 bZIP domain
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
12 months after purchase
This item will be shipped to you at ambient temperature in a lyophilised form.
1. Hai T, et al. Genes Dev. 3:2083-2090(1989).
2. Heilig R, et al. Nature 421:601-607(2003).
For research use only
NZ white rabbit
Polyclonal, whole serum
IHC, WB. Use at a dilution of 1 : 1000 for IHC-P and 1 : 2000 for WB. The optimal dilution should be determined by the end user. IMPORTANT: The lysate must be prepared in Ripa buffer containing 1% Triton-X100; 1% SDS and 1% SDC.
Specific for cFOS.
Rat, mouse, human. Other species not yet tested.
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.