Osenses Protocols 19-06-2016
G1 Lysing Buffer |
||
Component |
Amount |
Final Amount |
MQ water |
100 ml |
|
Tris (100 mM), pH 8.0 |
50 ml |
20 mM |
NaCl (5 M) |
7.5 ml |
150 mM |
EDTA (0.5 M) |
0.5 ml |
1 mM |
Triton-X100 |
2.5 ml |
1% |
Sodium Deoxycholate (MW: 414.55) |
2.5 g |
1% |
SDS |
2.5 g |
1% |
Protease Inhibitor from Roche with or without EDTA |
1 mini tablet |
|
MQ Water |
Up to 250 ml |
Homogenise 100 mg tissue in 1 ml cold lysing buffer.