|IHC-P on paraffin sections of rat brain.
The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.
Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm.
Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses.
Primary antibody: dilution 10 ug/ml, incubated 30 min at RT using Autostainer.
Sections were counterstained with Harris Hematoxylin.
|WB on tissue lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 5 ug/ml incubated overnight at 4C. Click on eLAB BOOK tab to see the details. Lysates were prepared in G1 lysing buffer containing 1% of: Triton X-100, SDS, SDC|
|APES coating of slides|
|SDS Loading Buffer|
|IHC-P on Autostainer|
|G1 Lysing Buffer|
|WB Protocol online|
|IHC-P HIER (Tris-EDTA, pH 9)|
|Davidson's fix (modified)|
|....Tissue lysate perparation|
|....G2 Lysing Buffer|
|....R Lysing Buffer|
|....G3 Lysing Buffer|
|....G4 Lysing Buffer|
|....G5 Lysing Buffer|
|.G6 Lysing Buffer|