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Code
OSC00183G
$240USD    Buy Now 

ID Tag
Rb2500-140415-WS

Immunogen
A synthetic peptide from human cFos protein conjugated to blue carrier protein has been used as the antigen. The peptide is homologous in many other species including rat, mouse, hamster and cat.

Accession

Also known
Proto-oncogene protein cFOS, c-Fos

Target
FUNCTION: Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation.
SUBUNIT: Heterodimer. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA. Interacts with MAFB
SUBCELLULAR LOCATION: Nucleus.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after purchase

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Hai T, et al. Genes Dev. 3:2083-2090(1989).

Limitation
For research use only

Related
products

Code
OSC00183G
$240USD    Buy Now 

Unit size
500 µg

Purity
Whole serum

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal antibody

Isotype
Polyclonal, whole serum

Applications
IHC, WB. Use at a concentration of 2-5 µg/ml. The optimal concentration should be determined by the end user. Not yet tested in other applications. For WB tissue lysates prepared in the G1 lysing buffer is recommended (G1 lysing buffer is Ripa buffer containing 1% Triton-X100; 1% SDS and 1% SDC).

Specificity
Specific for cFOS.

Spcs X-react.
Rat, mouse, marmoset, human. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Code
GSC00001P
$120USD   

ID Tag
563561-8

Unit size
50 ug

Accession

Also known
Proto-oncogene protein cFOS, c-Fos

Target
This is the immunising peptide for the products OSC00183G, OSR00004W, OSC00166W, OSC00007W, OSC00011G

Purity
>88%

Applications
Control peptide

Format
Lyophilised

Reconstitution
This peptide is insoluble.

Storage
Maintain refrigerated at 2-8C for up to 12 months. After reconstitution keep at -20C. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

Limitation
For research use only

Related Products
OSC00183G,OSR00004W,OSC00166W,OSC00007W,OSC00011G

Code
OSC00183G
$240USD    Buy Now 


Rabbit antibody to c Fos IHC-P on paraffin sections of rat brain.
The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.
Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm.
Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses.
Primary antibody: dilution 10 ug/ml, incubated 30 min at RT using Autostainer.
Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to c Fos WB on tissue lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 5 ug/ml incubated overnight at 4C. Click on eLAB BOOK tab to see the details. Lysates were prepared in G1 lysing buffer containing 1% of: Triton X-100, SDS, SDC
⚬ Products
Rabbit antibody to c Fos
Content

Download Protocols

APES coating of slides
SDS Loading Buffer
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
.G6 Lysing Buffer
Content

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