A synthetic peptide from human cFos protein conjugated to blue carrier protein has been used as the antigen. The peptide is homologous in many other species including rat and mouse.
Proto-oncogene protein cFOS, c-Fos
FUNCTION: Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation.
SUBUNIT: Heterodimer. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA. Interacts with MAFB
SUBCELLULAR LOCATION: Nucleus.
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
12 months after purchase
This item will be shipped to you at ambient temperature in a lyophilised form.
1. van Straaten F, et al. Proc. Natl. Acad. Sci. U.S.A. 80:3183-3187(1983).
For research use only
NZ white rabbit
Polyclonal, whole serum
IHC, WB. Use at a dilution of 1 : 1000 to 1 : 2000. The optimal dilution should be determined by the end user. Not yet tested in other applications. For WB, the lysate must be prepared in G1 lysing buffer which is: Ripa lysing buffer containing 1% Triton X-100; 1% SDS; 1% SDC.
Specific for cFos.
Rat, mouse, marmoset. Other species not yet tested.
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.