Osenses Protocols
5x Reducing Loading Buffer |
||
Component |
Add |
Final Amount |
Tris (2 M), pH 6.8 |
12.5 ml |
250 mM |
MQ water |
17 ml |
|
Glycerol |
50 ml |
50% |
SDS |
10 g |
10% |
2-ME |
12.5 ml |
12.5% |
Pink-Q colour (Osenses) |
Add to the lysate after heat denaturing. |
|
Total volume |
(add MQ water up to) 100 ml |
|
Add Tris, water and glycerol while stiring and heat upp the solution to 40-50C then slowly add the SDS and continue stirring (stop the heating), add the 2-ME and stir until the solution becomes translucent, it might take 1-2 hours. Apply heat/stir the solution until clear. Finally, adjust the volume with MQ water to 100 ml. Keep at Room Temperature for short term and for longer term keep at -20C. Pink-Q colour can be added after the sample has been heat-denatured. Add 1 ul per 100 ul of sample. |