Osenses Protocols

 

Materials

 

 

2% APES solution in Acetone

Component

FW

Amount

Final Concentration

APES (3-aminopropyltriethoxysilane; Sigma, A-3648)

 

15 ml

2% 

Acetone

 

Up to 750 ml

 

Prepare 2% fresh APES solution. 750 ml solution is enough to coat 700 slides. This solution is stable for 8 hours.

 

 

  1. Soak the slides in acetone for 2 minutes. Let them dry for 1-2 hours in the oven set at 60C.
  2. Dip the slides in the APES solution for 2 minutes.
  3. Wash the slides in 2 changes of MQ water (RO water also will be fine).
  4. Dry the slides in the oven set at 45C O/N.
  5. Store the treated slides in boxes at room temperature.