4% PFA |
||||
Component |
FW |
Amount |
Final Concentration |
pH |
MQ water |
|
1500 ml |
|
7.4 |
PBS (20x) |
|
100 ml |
|
|
Paraformaldehyde (Sigma, |
|
80 g |
4% |
|
NaOH |
|
18 pellets |
|
|
Adjust the pH to 7.4 and then the volume to 2000 ml with MQ water. Keep refrigerated. |
||||
Protocol
|