Osenses Protocols  01-07-2016

 

 

R Lysing Buffer

Component

Amount

Final Amount

MQ water

100 ml

 

Tris (100 mM), pH 8.0

50 ml

20 mM

NaCl (5 M)

7.5 ml

150 mM

EDTA (0.5 M)

0.5 ml

1 mM

Triton-X100

2.5 ml

1%

Sodium Deoxycholate (MW: 414.55)

1.25 g

0.5%

SDS

2.5 g

1%

Protease Inhibitor from Sigma

1 tablet

 

MQ Water

Up to 250 ml

 

Homogenise 100 mg tissue in 1 ml cold lysing buffer.