HIER: Tris-EDTA (TE buffer, pH 9.0)

Component

MW

Amount

Final Concentration

pH

RO H2O

 

1800 ml

 

9.0

Sigma 7-9 (equivalent to Tris base, T1378-1Kg)

121.14

2.42 g

10 mM

EDTA (0.5 M)

 

4 ml

1 mM

Stir and adjust the pH to 9.0 with Hcl.

RO H2O

Up to 2000 ml. Keep in cold room. The buffer should be clear and not cloudy.

Buffer pH is stable at 9.0 - 9.1, usually without adjustment. The buffer is stable for approximately 4 weeks at room temperature.