HIER: Tris-EDTA (TE buffer, pH 9.0) |
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Component |
MW |
Amount |
Final Concentration |
pH |
|
RO H2O |
|
1800 ml |
|
9.0 |
|
Sigma 7-9 (equivalent to Tris base, T1378-1Kg) |
121.14 |
2.42 g |
10 mM |
||
EDTA (0.5 M) |
|
4 ml |
1 mM |
||
Stir and adjust the pH to 9.0 with Hcl. |
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RO H2O |
Up to 2000 ml. Keep in cold room. The buffer should be clear and not cloudy. Buffer pH is stable at 9.0 - 9.1, usually without adjustment. The buffer is stable for approximately 4 weeks at room temperature. |