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Code
OSG00011G
$240USD    Buy Now 

ID Tag
Rb654-060908-G

Immunogen
A synthetic peptide to phospho S41 of human Axonal membrane protein GAP-43 conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in many species including rat, mouse and zebrafish.

Accession

Also known
Neuromodulin, growth-associated protein 43, protein F1, Basp2, B-50

Target
FUNCTION: This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons.
SUBUNIT: Binds calmodulin with a greater affinity in the absence of Ca(2+) than in its presence.
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse. Note: Cytoplasmic surface of growth cone and synaptic plasma membranes.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Cimler B.M, et al. J. Biol. Chem. 262:12158-12163(1987).
2. Ballif B.A, et al. Mol. Cell. Proteomics 3:1093-1101(2004).

Limitation
For research use only

Related
products

Code
OSG00011G
$240USD    Buy Now 

Unit size
500 µg

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
IgG

Clonality
Polyclonal

Isotype
Polyclonal, IgG

Applications
IHC, WB. Use at a concentration of 10-50 µg/ml. The optimal working concentration should be determined by the end user. Not yet tested in other applications. This antibody may also recognise the unphosphorylated form.

Specificity
Specific for GAP-43.

Spcs X-react.
Human, rat, mouse. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSG00011G
$240USD    Buy Now 


Rabbit antibody to phospho S41 GAP 43 WB on rat brain lysate using Rabbit antibody to phospho S41 GAP 43 (OSG00011G) at 50 µg/ml concentration incubated overnight at 4°C. Pre-absorption of the antibody with the immunising peptide completely abolishes the detected band.

⚬ Products
Rabbit antibody to phospho S41 GAP 43
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

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