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Code
OSG00010W
$240USD    Buy Now 

ID Tag
Rb654-060908-WS

Immunogen
A synthetic peptide to phospho S41 of human Axonal membrane protein GAP-43 conjugated to blue carrier protein was used as the antigen. The antigen is homologous in many species including rat, mouse and zebrafish.

Accession

Also known
Neuromodulin, growth-associated protein 43, protein F1, Basp2, B-50

Target
FUNCTION: This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons.
SUBUNIT: Binds calmodulin with a greater affinity in the absence of Ca(2+) than in its presence.
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse. Note: Cytoplasmic surface of growth cone and synaptic plasma membranes.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Cimler B.M, et al. J. Biol. Chem. 262:12158-12163(1987).

Limitation
For research use only

Related
products

Code
OSG00010W
$240USD    Buy Now 

Unit size
100 ul

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for GAP-43.

Spcs X-react.
Human, rat, mouse. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSG00010W
$240USD    Buy Now 


Rabbit antibody to phospho S41GAP 43 IHC on rat spinal cord (central canal) using Rabbit antibody to phospho S41GAP 43 at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).
Rabbit antibody to phospho S41GAP 43 IHC on rat spinal cord (dorsal horn) using Rabbit antibody to phospho S41GAP 43 at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).
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Rabbit antibody to phospho S41GAP 43
Content

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APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
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