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Code
OSS00040W
$240USD
ID Tag
Rb326-200308-WS
Immunogen
A synthetic peptide from aa region 2-50 of human SEC22b conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.
Accession
Also known
SEC22 vesicle-trafficking protein homolog B, SEC22 vesicle-trafficking protein-like 1, ERS24, ERS-24, Vesicle-trafficking protein SEC22b, SEC22L1, SEC22B
Target
The protein encoded by this gene is a member of the SEC22 family of vesicle trafficking proteins. It seems to complex with SNARE and it is thought to play a role in the ER-Golgi protein trafficking. This protein has strong similarity to Mus musculus and Cricetulus griseus proteins. There is evidence for use of multiple polyadenylation sites for the transcript.
FUNCTION: SNARE involved in targeting and fusion of ER-derived transport vesicles with the Golgi complex as well as Golgi-derived retrograde transport vesicles with the ER.
SUBUNIT: Component of two distinct SNARE complexes consisting of STX5, GOSR2/BOS1, BET1 and SEC22B or STX18, USE1L, BNIP1/SEC20L and SEC22B. YKT6 can probably replace SEC22B in either complex.
SUBCELLULAR LOCATION: Endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type IV membrane protein. Golgi apparatus membrane; Single-pass type IV membrane protein. Melanosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
FUNCTION: SNARE involved in targeting and fusion of ER-derived transport vesicles with the Golgi complex as well as Golgi-derived retrograde transport vesicles with the ER.
SUBUNIT: Component of two distinct SNARE complexes consisting of STX5, GOSR2/BOS1, BET1 and SEC22B or STX18, USE1L, BNIP1/SEC20L and SEC22B. YKT6 can probably replace SEC22B in either complex.
SUBCELLULAR LOCATION: Endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type IV membrane protein. Golgi apparatus membrane; Single-pass type IV membrane protein. Melanosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Mao M, et al. Proc. Natl. Acad. Sci. U.S.A. 95:8175-8180(1998).
2. Nakajima K, et al. EMBO J. 23:3216-3226(2004).
2. Nakajima K, et al. EMBO J. 23:3216-3226(2004).
Limitation
For research use only
Related
products
products
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Code
OSS00040W
$240USD
Unit size
100 µl
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user.
Specificity
Specific for SEC22B.
Spcs X-react.
Human, rat, mouse. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
OSS00040W
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OSS00040W
$240USD
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IHC on rat spinal cord using Rabbit antibody to SEC22B (OSS00040W) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown). |
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IHC on rat spinal cord using Rabbit antibody to SEC22B (OSS00040W) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown). |
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IHC on rat trigeminal using Rabbit antibody to SEC22B (OSS00040W) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown). |
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WB on rat brain using Rabbit antibody to SEC22B (OSS00040W) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the detected band. |
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IF on 1 day- old mouse cortical cell culture using Rabbit antibody to SEC22B (OSS00040W) at 1: 2000 dilution. DAPI counter stained. |
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IHC on rat spinal cord using Rabbit antibody to SEC22B (OSS00040W) at 1 : 3000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown). |
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IHC on rat spinal cord using Rabbit antibody to SEC22B (OSS00040W) at 1 : 3000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown). |
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IHC on rat brain using Rabbit antibody to SEC22B (OSS00040W) at 1 : 3000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown). |
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IHC on rat brain using Rabbit antibody to SEC22B (OSS00040W) at 1 : 3000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown). |
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IHC on rat brain using Rabbit antibody to SEC22B (OSS00040W) at 1 : 3000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown). |
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IHC on rat spinal cord using Rabbit antibody to SEC22B (OSS00040W) at 1 : 3000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown). |
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IHC on rat spinal cord using Rabbit antibody to SEC22B (OSS00040W) at 1 : 3000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown). |
⚬ Products
Rabbit antibody to SEC22B (2-50)
