A synthetic peptide from mouse VGluT2 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat.
Vesicular glutamate transporter 2, VGlU2, Solute carrier family 17 member 6, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, Differentiation-associated BNPI, Slc17a6, Dnpi
FUNCTION: Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.
SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Membrane; Multi-pass membrane protein. Cell junction, synapse, synaptosome.
TISSUE SPECIFICITY: Expressed in brain. Expressed in hippocampal neurons (at protein level).
DEVELOPMENTAL STAGE: Expressed in brain throughout development. Transiently expressed in hippocampal neurons during the first week after birth, with expression decreasing thereafter.
MISCELLANEOUS: Mice lacking this protein exhibit an elevated rate of perinatal lethality. Surviving animals display a strong reduction in evoked glutamergic responses in thalamic neurons. Reduction of protein level in homozygous and heterozygous knockouts leads to a graded reduction in the amplitude of the postsynaptic response to single vesicle fusion in thalamic neurons, consistent with a role for this protein in determining quantal size.
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
12 months after reconstitution
This item will be shipped to you at ambient temperature in a lyophilised form.
1. Li JL, et al. J Comp Neurol 457:236-49 (2003).
For research use only
Polyclonal, whole serum
IHC, WB. A dilution of 1: 500 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specific for VGLUT2.
Rat, mouse, marmoset. Other species not yet tested.
Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material.