A synthetic peptide from mouse ChAT conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and human.
CHOACTase, choline acetylase, choline O-acetyltransferase, CMS1A, CMS1A2
Cholinergic systems are implicated in numerous neurologic functions. Alteration in some cholinergic neurons may account for the disturbances of Alzheimer disease. The protein encoded by this gene synthesizes the neurotransmitter acetylcholine. Alternative splice variants have been found that contain alternative 5' untranslated exons. Three of the four described splice variants encode identical 69 kDa proteins while one variant encodes both the 69 kDa and a larger 82 kDa protein.
Choline acetylase catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
CATALYTIC ACTIVITY: Acetyl-CoA + choline = CoA + O-acetylcholine.
Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2); also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Inheritance is autosomal recessive.
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
12 months after reconstitution
This item will be shipped to you at ambient temperature in a lyophilised form.
1. Ishii K, et al. Brain Res. Mol. Brain Res. 7:151-159(1990).
For research use only
NZ white rabbit
Polyclonal, whole serum
IHC, WB. A dilution of 1: 250 is recommended for IHC-P and 1:500 for WB. The optimal dilution should be determined by the end user.
Specific for ChAT.
Rat, mouse, marmoset, human. Other species not yet tested.
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.