⚬ Services
Code
OSM00145W
$240USD    Buy Now 

ID Tag
Rb2537-230615-WS

Immunogen
A synthetic peptide from aa region 50-100 of human RHOT1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.

Accession

Also known
Mitochondrial Rho GTPase 1, MIRO-1, Rac-GTP-binding protein-like protein, Ras homolog gene family member T1, ARHT1, MIRO1, MIRO 1

Target
Function: Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution.
Subcellular location: Mitochondrion outer membrane; Single-pass type IV membrane protein.
Note: Colocalizes with MGARP and RHOT2 at the mitochondria.
Tissue specificity: Ubiquitously expressed. Expressed at high level in heart and skeletal muscle

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Fransson A, et al. J. Biol. Chem. 278:6495-6502(2003)

Limitation
For research use only

Code
OSM00145W
$240USD    Buy Now 

Unit size
100 µl

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1 : 1000 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for RHOT1.

Spcs X-react.
Human, mouse, rat. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSM00145W
$240USD    Buy Now 


Rabbit antibody to RHOT1 (50-100) WB on rat spinal cord lysate. Blocking with 0.5% LFDM for 30 min at RT; Primary antibody used at 1:1000 dilution incubated overnight at 4C. Click on eLAB BOOK tab to see the details.

⚬ Products
Rabbit antibody to RHOT1 (50-100)
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

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