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Code
OST00344W
$240USD    Buy Now 

ID Tag
Rb1344-140310-WS

Immunogen
A synthetic peptide from aa region 160-210 of mouse TRIM32 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and shares 95% identity with human sequence.

Accession

Also known
TRI32, Tripartite motif-containing protein 32, TRIM32

Target
Function: Has an E3 ubiquitin ligase activity. Ubiquitinates DTNBP1 (dysbindin). Ubiquitinates PIAS4/PIASY and promotes its degradation in keratinocytes treated with UVB and TNF-alpha.
Subcellular location: Cytoplasm. Note: Localized in cytoplasmic bodies, usually concentrated around the nucleus.
Tissue specificity: Ubiquitous. High expression in brain.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Horn E.J, et al. Carcinogenesis 25:157-167(2004)
2. Albor A, et al. J. Biol. Chem. 281:25850-25866(2006)

Limitation
For research use only

Code
OST00344W
$240USD    Buy Now 

Unit size
100 µl

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1: 1000 to 1 : 3000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for TRIM32.

Spcs X-react.
Mouse, rat. Other species not yet tested but expected to work in human.

Format
Lyophilised

Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OST00344W
$240USD    Buy Now 


Rabbit antibody to TRIM32 (160-210) IHC-P on paraffin sections of rat spinal cord. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to TRIM32 (160-210) WB on mouse muscle lysate. Blocking with 1% LFDM for 30 min at RT; Primary antibody used at 1:1000 dilution incubated overnight at 4C. Click on eLAB BOOK tab to see the details.
Rabbit antibody to TRIM32 (160-210) IHC-P on paraffin sections of rat spinal cord. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.
⚬ Products
Rabbit antibody to TRIM32 (160-210)
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

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