A synthetic peptide from aa region 500-550 of human ATP5A1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.
ATPA, mitochondrial ATP synthase subunit alpha, ATP synthase subunit alpha, mitochondrial, ATP5A, ATP5AL2, ATPM
FUNCTION: Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F1. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites.
Subcellular location: Mitochondrion inner membrane. Note: Peripheral membrane protein.
Tissue specificity: Fetal lung, heart, liver, gut and kidney. Expressed at higher levels in the fetal brain, retina and spinal cord.
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
12 months after reconstitution
This item will be shipped to you at ambient temperature in a lyophilised form.
1. Kataoka H, et al. Biochim. Biophys. Acta 1089:393-395(1991)
For research use only
NZ white rabbit
Polyclonal, whole serum
IHC, WB. A dilution of 1: 2000 for IHC-P and 1:3000 for WB is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specific for ATP5A1.
Human, mouse, rat. Other species not yet tested.
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.