A synthetic peptide from aa region 100-150 of human contactin 1 conjugated to an immunogenic carrier protein was used as the antigen.
Neural cell surface protein F3, F3cam, contactin 1, CNTN1, Glycoprotein gp135
FUNCTION: Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth.
SUBUNIT: Monomer. Interacts with CNTNAP1 in cis form. Binds to the carbonic-anhydrase like domain of protein-tyrosine phosphatase zeta. Interacts with NOTCH1 and TNR.
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor.
MISCELLANEOUS: F3 shares with L1, N-CAM, MAG, and other cell adhesion molecules from nervous tissue the L2/HNK-1 carbohydrate epitope.
TISSUE SPECIFICITY: Strongly expressed in brain and in neuroblastoma and retinoblastoma cell lines. Lower levels of expression in lung, pancreas, kidney and skeletal muscle.
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
12 months after reconstitution
This item will be shipped to you at ambient temperature in a lyophilised form.
1. Reid R.A, et al. Brain Res. Mol. Brain Res. 21:1-8(1994).
2. Sjoeblom T, et al. Science 314:268-274(2006).
For research use only
NZ white rabbit
IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.
Specific for contactin-1.
Human, rat. Other species not yet tested.
Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.