⚬ Services
Code
OSC00026W
$240USD    Buy Now 

ID Tag
Rb429-060408-WS

Immunogen
A synthetic peptide from mouse Contactin-1 (contactin 1, CNTN1) conjugated to an immunogenic carrier protein was used as the antigen.

Accession

Also known
Neural cell surface protein F3, F3cam, contactin 1, CNTN1

Target
FUNCTION: Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth.
SUBUNIT: Monomer. Interacts with CNTNAP1 in cis form. Binds to the carbonic-anhydrase like domain of protein-tyrosine phosphatase zeta. Interacts with NOTCH1 and TNR.
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor.
MISCELLANEOUS: F3 shares with L1, N-CAM, MAG, and other cell adhesion molecules from nervous tissue the L2/HNK-1 carbohydrate epitope.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Gennarini G, et al. J. Cell Biol. 109:775-788(1989).
2. Hu Q.-D, et al. Cell 115:163-175(2003).

Limitation
For research use only

Related
products
 
Code Product Name
OSC00027W Rabbit antibody to Contactin 1
 

Code
OSC00026W
$240USD    Buy Now 

Unit size
100 µl

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for contactin-1.

Spcs X-react.
Mouse. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSC00026W
$240USD    Buy Now 


Rabbit antibody to Contactin 1 IHC on rat brain (paraffin section) using Rabbit antibody to mouse contactin-1 (Contactin 1, CNTN1): whole serum (OSC00026W) at 1 : 300 dilution incubated overnight at 4C, developed with ABC/DAB/Ni.
Rabbit antibody to Contactin 1 IHC on rat brain (paraffin section) using Rabbit antibody to mouse contactin-1 (Contactin 1, CNTN1): whole serum (OSC00026W) at 1 : 300 dilution incubated overnight at 4C, developed with ABC/DAB/Ni.
Rabbit antibody to Contactin 1 IHC on rat brain (paraffin section) using Rabbit antibody to mouse contactin-1 (Contactin 1, CNTN1): whole serum (OSC00026W) at 1 : 300 dilution incubated overnight at 4C, developed with ABC/DAB/Ni.
⚬ Products
Rabbit antibody to Contactin 1
Content

Download Protocols

APES coating of slides
SDS Loading Buffer
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
.G6 Lysing Buffer
Content

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