⚬ Services
Code
OSR00316W
$240USD    Buy Now 

ID Tag
Rb789-140209-WS

Immunogen
A synthetic peptide from aa region 130-180 of human RhoF conjugated to blue carrier protein was used as the antigen. The antigen is homologous in rat and mouse.

Accession

Also known
Rho family GTPase Rif, Rho in filopodia, ARHF, RIF, Rho in filopodia

Target
Function: Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Causes the formation of thin, actin-rich surface projections called filopodia. Functions cooperatively with CDC42 and Rac to generate additional structures, increasing the diversity of actin-based morphology.
Subcellular location: Cell membrane; Lipid-anchor; Cytoplasmic sidePotential. Cytoplasm › cytoskeleton.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Ellis S, et al. Curr. Biol. 10:1387-1390(2000)
2. Ota T, et al. Nat. Genet. 36:40-45(2004)

Limitation
For research use only

Related
products

Code
OSR00316W
$240USD    Buy Now 

Unit size
100 µl

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for RhOF.

Spcs X-react.
Human, rat, mouse. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSR00316W
$240USD    Buy Now 


Rabbit antibody to RhoF (130-180) WB on rat spinal cord  lysate using Rabbit antibody to RhoF (130-180) (OSR00316W) at 1:500 dilution. Incubated 30 min at RT with shake. Blocking: 0.5% LFDM in 1x PBS containing 0.1% Tween-20

⚬ Products
Rabbit antibody to RhoF (130-180)
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

Watch Videos

Autoperfuser™ OS2100
Red Carpet: A Histology Carpet for Slides