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Code
OSV00035G
$240USD
ID Tag
Rb699-060908-G
Immunogen
A synthetic peptide from human VDP conjugated to blue carrier protein was used as the antigen. The antigen is homologous in rat and moue.
Accession
Also known
Protein USO1 homolog, Transcytosis-associated protein, TAP, General vesicular transport factor p115, USO1, VDP
Target
FUNCTION: General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.
SUBCELLULAR LOCATION: Cytoplasm, cytosol. Golgi apparatus membrane; Peripheral membrane protein. Note: Recycles between the cytosol and the Golgi apparatus during interphase.
SUBCELLULAR LOCATION: Cytoplasm, cytosol. Golgi apparatus membrane; Peripheral membrane protein. Note: Recycles between the cytosol and the Golgi apparatus during interphase.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Sohda M, et al. J. Biol. Chem. 273:5385-5388(1998).
2. Han G, et al. Proteomics 8:1346-1361(2008).
2. Han G, et al. Proteomics 8:1346-1361(2008).
Limitation
For research use only
Related
products
products
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Code
OSV00035G
$240USD
Unit size
500 µg
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
IgG
Clonality
Polyclonal
Isotype
Polyclonal, IgG
Applications
IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for VDP.
Spcs X-react.
Human, Mouse, rat. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
OSV00035G
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OSV00035G
$240USD
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Human Melanoma cell line C 32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and washed twice with PBS before fixing with 2% formalin for 10 minutes. Cells were then washed three times with PBS and incubated with Tris 0.01M containing Triton X 0.005% for 15 minutes. Cells were washed and incubated with 100 µl of Rabbit antibody to internal region of Vesicle docking protein (VDP, USO1, TAP, Transcytosis-associated protein): IgG (OSV00035G) diluted 1:100 in the blocking buffer for 30 minutes. Welles were then washed 7 times with PBS and incubated with 100 µl of anti Rb-FITC conjugate diluted 1:100 in the blocking buffer for further 30 minutes. Cells were washed as before and nuclear counter stained with Hoechst and mounted on to slides. |
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Human Melanoma cell line C 32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and washed twice with PBS before fixing with 2% formalin for 10 minutes. Cells were then washed three times with PBS and incubated with Tris 0.01M containing Triton X 0.005% for 15 minutes. Cells were washed and incubated with 100 µl of Rabbit antibody to internal region of Vesicle docking protein (VDP, USO1, TAP, Transcytosis-associated protein): IgG (OSV00035G) diluted 1:100 in the blocking buffer for 30 minutes. Welles were then washed 7 times with PBS and incubated with 100 µl of anti Rb-FITC conjugate diluted 1:100 in the blocking buffer for further 30 minutes. Cells were washed as before and nuclear counter stained with Hoechst and mounted on to slides. |
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Human Melanoma cell line C 32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and washed twice with PBS before fixing with 2% formalin for 10 minutes. Cells were then washed three times with PBS and incubated with Tris 0.01M containing Triton X 0.005% for 15 minutes. Cells were washed and incubated with 100 µl of Rabbit antibody to internal region of Vesicle docking protein (VDP, USO1, TAP, Transcytosis-associated protein): IgG (OSV00035G) diluted 1:100 in the blocking buffer for 30 minutes. Welles were then washed 7 times with PBS and incubated with 100 µl of anti Rb-FITC conjugate diluted 1:100 in the blocking buffer for further 30 minutes. Cells were washed as before and nuclear counter stained with Hoechst and mounted on to slides. |
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Human Melanoma cell line C 32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and washed twice with PBS before fixing with 2% formalin for 10 minutes. Cells were then washed three times with PBS and incubated with Tris 0.01M containing Triton X 0.005% for 15 minutes. Cells were washed and incubated with 100 µl of Rabbit antibody to internal region of Vesicle docking protein (VDP, USO1, TAP, Transcytosis-associated protein): IgG (OSV00035G) diluted 1:100 in the blocking buffer for 30 minutes. Welles were then washed 7 times with PBS and incubated with 100 µl of anti Rb-FITC conjugate diluted 1:100 in the blocking buffer for further 30 minutes. Cells were washed as before and nuclear counter stained with Hoechst and mounted on to slides. |
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