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Code
OSV00018W
$240USD
ID Tag
Rb699-060908-WS
Immunogen
A synthetic peptide from the internal region of human VDP115 conjugated to blue carrier protein was used as the antigen. The antigen is homologous in rat and moue.
Accession
Also known
Protein USO1 homolog, Transcytosis-associated protein, TAP, General vesicular transport factor p115, USO1, VDP
Target
FUNCTION: General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.
SUBCELLULAR LOCATION: Cytoplasm, cytosol. Golgi apparatus membrane; Peripheral membrane protein. Note: Recycles between the cytosol and the Golgi apparatus during interphase.
SUBCELLULAR LOCATION: Cytoplasm, cytosol. Golgi apparatus membrane; Peripheral membrane protein. Note: Recycles between the cytosol and the Golgi apparatus during interphase.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Sohda M, et al. J. Biol. Chem. 273:5385-5388(1998).
2. Olsen J.V, et al. Cell 127:635-648(2006).
2. Olsen J.V, et al. Cell 127:635-648(2006).
Limitation
For research use only
Related
products
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Code
OSV00018W
$240USD
Unit size
100 µl
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user.
Specificity
Specific for VDP.
Spcs X-react.
Human, mouse, rat. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
OSV00018W
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OSV00018W
$240USD
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Human Melanoma cell line C 32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and washed twice with PBS before fixing with 2% formalin for 10 minutes. Cells were then washed three times with PBS and incubated with Tris 0.01M containing Triton X 0.005% for 15 minutes. Cells were washed and incubated with 100 µl of Rabbit antibody to internal region of Vesicle docking protein (VDP, USO1, TAP, Transcytosis-associated protein): whole serum (OSV00018W) diluted 1:100 in the blocking buffer for 30 minutes. Welles were then washed 7 times with PBS and incubated with 100 µl of anti Rb-FITC conjugate diluted 1:100 in the blocking buffer for further 30 minutes. Cells were washed as before and nuclear counter stained with Hoechst and mounted on to slides. |
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Human Melanoma cell line C 32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and washed twice with PBS before fixing with 2% formalin for 10 minutes. Cells were then washed three times with PBS and incubated with Tris 0.01M containing Triton X 0.005% for 15 minutes. Cells were washed and incubated with 100 µl of Rabbit antibody to internal region of Vesicle docking protein (VDP, USO1, TAP, Transcytosis-associated protein): whole serum (OSV00018W) diluted 1:100 in the blocking buffer for 30 minutes. Welles were then washed 7 times with PBS and incubated with 100 µl of anti Rb-FITC conjugate diluted 1:100 in the blocking buffer for further 30 minutes. Cells were washed as before and nuclear counter stained with Hoechst and mounted on to slides. |
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Human Melanoma cell line C 32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and washed twice with PBS before fixing with 2% formalin for 10 minutes. Cells were then washed three times with PBS and incubated with Tris 0.01M containing Triton X 0.005% for 15 minutes. Cells were washed and incubated with 100 µl of Rabbit antibody to internal region of Vesicle docking protein (VDP, USO1, TAP, Transcytosis-associated protein): whole serum (OSV00018W) diluted 1:100 in the blocking buffer for 30 minutes. Welles were then washed 7 times with PBS and incubated with 100 µl of anti Rb-FITC conjugate diluted 1:100 in the blocking buffer for further 30 minutes. Cells were washed as before and nuclear counter stained with Hoechst and mounted on to slides. |
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1. Fixation: Formalin 2% for 15 minutes 2. Wash once 3. Permeabilized with: 0.05% Triton X glycine 0.01M 4. Blocked with 5% FCS in the buffer above for 20 minutes 5. Primary antibody 1:200 6. Incubated for 2h room temp 7. Wash 3X 10 min each 8. Secondary antibody mlecular probe alexa 488 diluted 1:300 9. Incubate for 2 hours 10. In the last 30 min counter stain with Phalloiding 5 ug mL and Hoertsch 11. Wash three time as above |
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