A synthetic peptide from human Beclin1 conjugated to blue carrier protein has been used as the antigen. The peptide is homologous in rat and mouse.
Beclin-1, BECN1, GT197, ATG6, VPS30, beclin1
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. It may play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus. May play a role in autophagy.
SUBCELLULAR LOCATION: Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Note: Expressed in dendrites and cell bodies of cerebellar Purkinje cells
TISSUE SPECIFICITY: Ubiquitous
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
12 months after reconstitution
This item will be shipped to you at ambient temperature in a lyophilised form.
1. Feng W, et al. J. Mol. Biol. 372 (1), 223-235(2007).
For research use only
NZ white rabbit
Polyclonal, whole serum
IHC, WB. Use at a dilution of 1 : 2000. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specific for Beclin-1.
Human, mouse, rat, marmoset. Other species not yet tested.
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.