⚬ Services
   
Guinea pig antibody to ChAT

$240USD 

Code OSC00334W

eLab Book

Osenses Protocols: Western Blot

 

.................................................................................................................................................................................

 

1. Run the SDS-PAGE at 250V.

 

2. Soak the gel for 10 min in transfer buffer then rinse it with MQ water.

 

3. Soak the membrane for 5 min in MQ water.

 

4. Semi-dry transfer at 10 V.

5. After transfer, rinse once the membrane with PBS and then wash 3x, 5 min each with 2xPBS in 100 ml. Shake at 50 RPM.

 

6. Block with 1xPBS containing 1% LFDM for 30 min at RT with shake at 50 RPM.

 

7. Rinse with 70 ml of PBS.

 

8. Incubate O/N in the fridge (3-5C).

 

9. Wash the membrane 5 times (5 min each) with PBST.  Wash in 100 ml with shake 50 RPM.

 

10. Add the Osenses Rabbit anti Gp adaptobody at 1:3000 dilution, rinse and then add Sigma HRP-Goat anti Rb (diluted 1: 20,000). Add 6 ml per membrane (6x8 cm). Incubate 30 min to 1 hour at RT with 50 RPM shake.

 

11. Wash 4 times (5min each) with shake 50 RPM with 100 ml of 200 mM NaCl-T containing 0.1% Tween-20.

 

12. Wash 6 times more with 100 ml of PBS with shake 50 RPM.

 

13. Prepare the ECL substrate BioRad.

 

14. Scan the blot on C-Digit at High resolution (12 minutes).

 


   
⚬ Products
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

Watch Videos

Autoperfuser™ OS2100
Red Carpet: A Histology Carpet for Slides