A synthetic peptide from rat TRPA1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse.
The location of the epitope is highlighted in
Transient receptor potential cation channel subfamily A member 1, ankyrin-like with transmembrane domains protein 1, transformation sensitive protein p120, ANKTM1
The structure of the protein encoded by this gene is highly related to both the protein ankyrin and transmembrane proteins. The specific function of this protein has not yet been determined; however, studies indicate the function may involve a role in signal transduction and growth control.
TISSUE SPECIFICITY: Expressed in inner ear. In inner ear, it is found in the distal half of stereocilia, along the length of the kinocilium and at high level in the pericuticular zone (at protein level). Specifically expressed in a subset of nociceptive neurons. Expressed in the same neurons that TRPV1. In contrast, it is not expressed in neurons expressing TRPM8. Expressed in the superior cervical ganglion.
DEVELOPMENTAL STAGE: During utricle development, it is first expressed at E15 and E16 and peaks at E17. It drops at E18 but increases again at E19, possibly corresponding to a second wave of hair cells that are generated at E15.
Inhibited by ruthenium red, a potent blocker of TRPV channels.
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
12 months after reconstitution
This item will be shipped to you at ambient temperature in a lyophilised form.
1. Jordt S.-E, et al. Nature 427:260-265(2004).
For research use only
NZ white rabbit
Polyclonal, whole serum
IHC, WB. A dilution of 1 : 1000 is recommended for WB and 1:2000 for IHC-P. The optimal dilution should be determined by the end user. Not yet tested in other applications. For WB, tissue lysates should be prepared in G3 lysing buffer (G3 lysing buffer contains only 1% Triton X-100 and no other detergents).
Specific for TRPA1.
Rat, mouse, human, marmoset. Other species not yet tested.
Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material.