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Code
OSR00269W
$240USD
ID Tag
Rb329-200308-WS
Immunogen
A synthetic peptide from aa region 100-150 of of human RAC1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat, mouse, guinea pig, bovine, xenopus, dog, zebrafish and chicken.
Accession
Also known
RAC1 is also known as: p21-Rac1, Ras-like protein TC25, Cell migration-inducing gene 5 protein; RAC2 is also known as: p21-Rac2, Small G protein, GX
Target
FUNCTION: Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.
ENZYME REGULATION: Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) which increase the GTP hydrolysis activity, and GDP dissociation inhibitors which inhibit the dissociation of the nucleotide from the GTPase.
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side. Melanosome. Note: Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
RAC1:
TISSUE SPECIFICITY: Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. The expression of isoform B is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.
RAC2:
TISSUE SPECIFICITY: Hematopoietic specific.
DISEASE: Defects in RAC2 are the cause of neutrophil immunodeficiency syndrome.
ENZYME REGULATION: Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) which increase the GTP hydrolysis activity, and GDP dissociation inhibitors which inhibit the dissociation of the nucleotide from the GTPase.
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side. Melanosome. Note: Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
RAC1:
TISSUE SPECIFICITY: Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. The expression of isoform B is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.
RAC2:
TISSUE SPECIFICITY: Hematopoietic specific.
DISEASE: Defects in RAC2 are the cause of neutrophil immunodeficiency syndrome.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Didsbury J, et al. J. Biol. Chem. 264:16378-16382(1989).
Limitation
For research use only
Code
OSR00269W
$240USD
Unit size
100 ul
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB. A dilution of 1 : 1000 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for RAC1 and RAC2.
Spcs X-react.
Human, rat, mouse. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
- Click to see the eLab Book
Code
OSR00269W
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OSR00269W
$240USD
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WB on tissue lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody dilution: 1:1000 incubated overnight at 4C. Click on eLAB BOOK tab to see the details. |
⚬ Products
Rabbit antibody to RAC1, RAC2 (100-150)
