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Code
OSC00025W
$240USD
ID Tag
Rb332-200308-WS
Immunogen
A synthetic peptide from human CDC42, aa region of 50-100 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in many other species.
Accession
Also known
Rho-related GTP-binding protein RhOA, RhOB, RhOC, RhOF, RhOQ, RAC1, RAC2, RAC3, CDC42
Target
The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Nakamura T, et al. Biochem. Biophys. Res. Commun. 226:688-694(1996).
2. Westmark C.J, et al. Oncogene 24:502-511(2005).
2. Westmark C.J, et al. Oncogene 24:502-511(2005).
Limitation
For research use only
Related
products
products
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Code
OSC00025W
$240USD
Unit size
100 ul
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for RhO family.
Spcs X-react.
Human, rat, mouse. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
OSC00025W
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OSC00025W
$240USD
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WB on rat brain lysate using Rabbit antibody to pan RhO, RAC, CDC42 (OSC00025W) at 1 : 1000 dilution. |
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IHC on rat midbrain using Rabbit antibody to pan RhO, RAC, CDC42 (OSC00025W) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown). |
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IF on 1 day- old mouse cortical cell culture using Rabbit antibody to pan RhO, RAC, CDC42 (OSC00025W) at 1: 2000 dilution. DAPI counter stained. |
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IF on 1 day- old mouse cortical cell culture using Rabbit antibody to pan RhO, RAC, CDC42 (OSC00025W) at 1: 2000 dilution. DAPI counter stained. |
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