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Code
OSC00009W
$240USD    Buy Now 

ID Tag
Rb259-270108-WS

Immunogen
A synthetic peptide from human Cathelicidin conjugated to blue carrier protein has been used as the antigen.
The location of the epitope is highlighted in green.

Accession

Also known
CAMP, CAP18, FALL39, FALL-39, 18 kDa cationic antimicrobial protein, CAP-18, hCAP-18, LL37, CRAMP, HSD26

Target
Cathelicidin antimicrobial protein is an antimicrobial protein found in specific granules of polymorphonuclear leukocytes.
FUNCTION: Binds to bacterial lipopolysaccharides (LPS), has antibacterial activity.
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Expressed in bone marrow and testis and neutrophils.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Gambichler T et al. Br J Dermatol 161:1136-42 (2009)

Limitation
For research use only

Code
OSC00009W
$240USD    Buy Now 

Unit size
150 µl

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. Use at a dilution of 1: 1000. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for Cathelicidin antimicrobial peptide.

Spcs X-react.
Human, other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 150 µl of sterile water. Centrifuge to remove any insoluble material.

Code
GSC00002P
$120USD   

ID Tag
68912-103

Unit size
50 µg

Accession

Also known
CAMP, CAP18, FALL39, FALL-39, 18 kDa cationic antimicrobial protein, CAP-18, hCAP-18, LL37, CRAMP, HSD26

Target
This is the immunising peptide for the product Rabbit antibody to human Cathelicidin antimicrobial peptide (CAP-18, hCAP-18, antibacterial protein LL-37, CAMP, CRAMP, FALL39): whole serum (OSC00009W).

Purity
>94%

Applications
Control peptide

Format
Lyophilised

Reconstitution
This peptide is soluble in PBS and water.

Storage
Maintain refrigerated at 2-8°C for up to 12 months. After reconstitution keep at -20°C. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

Limitation
For research use only

Related Products
OSC00009W,OSC00028G

Code
OSC00009W
$240USD    Buy Now 


Rabbit antibody to LL-37 IHC on paraffin sections of human large intestine tissue using Rabbit antibody to LL-37: OSC00009W.
HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module.
Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm.
Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions.
Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer)
Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to LL-37 IF on ethanol-fixed human spermatosoid using Rabbit antibody to LL-37 (OSC00009W) at 1: 100 dilution.
Rabbit antibody to LL-37 IHC on paraffin sections of human large intestine tissue using Rabbit antibody to LL-37: OSC00009W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to LL-37 IHC on paraffin sections of human lung tissue using Rabbit antibody to LL-37: OSC00009W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to LL-37 IHC on paraffin sections of human kidney tissue using Rabbit antibody to LL-37: OSC00009W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to LL-37 IHC on paraffin sections of human fallopian tube tissue using Rabbit antibody to LL-37: OSC00009W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to LL-37 IHC on paraffin sections of human appendix tissue using Rabbit antibody to LL-37: OSC00009W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to LL-37 IHC on paraffin sections of human liver tissue using Rabbit antibody to LL-37: OSC00009W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacture\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to LL-37 IHC on paraffin sections of human liver tissue using Rabbit antibody to LL-37: OSC00009W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to LL-37 IHC on paraffin sections of human placenta tissue using Rabbit antibody to LL-37: OSC00009W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to LL-37 IHC on paraffin sections of human prostate tissue using Rabbit antibody to LL-37: OSC00009W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to LL-37 IHC on paraffin sections of human skin tissue using Rabbit antibody to LL-37: OSC00009W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to LL-37 IHC on paraffin sections of human thyroid tissue using Rabbit antibody to LL-37: OSC00009W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to LL-37 IHC on paraffin sections of human tonsil tissue using Rabbit antibody to LL-37: OSC00009W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to LL-37 IHC on paraffin sections of human tonsil tissue using Rabbit antibody to LL-37: OSC00009W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to LL-37 IHC on paraffin sections of human tonsil tissue using Rabbit antibody to LL-37: OSC00009W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to LL-37 IHC on paraffin sections of human tonsil tissue using Rabbit antibody to LL-37: OSC00009W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to LL-37 Staining of a cytospin preparation of peripheral blood mononuclear cells isolated from buffycoat. Cells were left to air dry and then fixed with cold acetone (90 seconds) and blocked with PBS containing 1% FCS and 0.1% saponin (blocking buffer) for 20 minutes. Cells were then washed twice in PBS and incubated with Rabbit antibody to LL-37 (OSC00009W), diluted 1 : 200 in blocking buffer, for 1 hour at room temperature. Cells were washed twice in PBS followed by incubation for 30 minutes with the FITC-labeled secondary antibody. Finally, cells were washed and nuclear counterstained with DAPI. The antibody selectively recognizes polymorphonuclar cells.
Rabbit antibody to LL-37 WB on different lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:1000 incubated at 4C overnight. Click on eLAB BOOK tab to see the details.
⚬ Products
Rabbit antibody to LL-37
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Download Protocols

APES coating of slides
SDS Loading Buffer
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
.G6 Lysing Buffer
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