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Code
OSA00012G
$240USD
ID Tag
Sh12-281007-G
Immunogen
A synthetic peptide from c-terminal region of human ATG7 conjugated to blue carrier protein has been used as the antigen. The peptide is homologous in rat and mouse.
Accession
Also known
ATG7, APG7-like, APG7L, hAGP7, APG7L, ubiquitin-activating enzyme E1-like protein
Target
This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vaculuolar membranes. The protein shows homlogy to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes.
FUNCTION: Functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. Forms intermediate conjugates with GABARAPL1 (GABARAPL2, GABARAP or MAP1ALC3). Formation of the final GABARAPL1-PE conjugate is essential for autophagy.
SUBUNIT: Homodimer. Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.
DOMAIN: The C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12.
FUNCTION: Functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. Forms intermediate conjugates with GABARAPL1 (GABARAPL2, GABARAP or MAP1ALC3). Formation of the final GABARAPL1-PE conjugate is essential for autophagy.
SUBUNIT: Homodimer. Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.
DOMAIN: The C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Tanida I, et al. FEBS J. 273 (11), 2553-2562(2006).
Limitation
For research use only
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Code
OSA00012G
$240USD
Unit size
500 ug
Conjugate
Unconjugated antibody
Host
Sheep
Purity
IgG
Clonality
Polyclonal
Isotype
IgG
Applications
IHC, WB. Use at a concentration of 10-50 ug/ml. The optimal working concentration should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for ATG7.
Spcs X-react.
Human, mouse and rat. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 500 ul of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
OSA00012G
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
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Sheep antibody to ATG7
