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Code
OSA00011G
$240USD
ID Tag
Sh6-281007-G
Immunogen
A synthetic peptide from human ATG3 conjugated to an immunogenic carrier protein has been used as the antigen. The antigen is homologous in rat and mouse.
Accession
Also known
APG3, APG3L, APG3-like
Target
Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3, a protein-conjugating enzyme essential for autophagy.
FUNCTION: GABARAPL1 (GABARAPL2 or GABARAP or MAP1LC3)-modifier protein conjugating enzyme involved in its E2-like covalent binding to PE. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3 (E2-like enzyme). Preferred substrate is MAP1LC3A. Formation of the GABARAPL1-PE conjugate is essential for autophagy.
SUBCELLULAR LOCATION: Cytoplasm
TISSUE SPECIFICITY: Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta.
FUNCTION: GABARAPL1 (GABARAPL2 or GABARAP or MAP1LC3)-modifier protein conjugating enzyme involved in its E2-like covalent binding to PE. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3 (E2-like enzyme). Preferred substrate is MAP1LC3A. Formation of the GABARAPL1-PE conjugate is essential for autophagy.
SUBCELLULAR LOCATION: Cytoplasm
TISSUE SPECIFICITY: Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Tanida I, et al. FEBS J. 273 (11), 2553-2562(2006).
2. Brill L.M, et al. Anal. Chem. 76 (10), 2763-2772(2004).
2. Brill L.M, et al. Anal. Chem. 76 (10), 2763-2772(2004).
Limitation
For research use only
Related
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Code
OSA00011G
$240USD
Unit size
500 µg
Conjugate
Unconjugated antibody
Host
Sheep
Purity
IgG
Clonality
Polyclonal
Isotype
IgG
Applications
IHC, WB. Use at a concentration of 10-50 µg/ml. The optimal working concentration should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for ATG3.
Spcs X-react.
Human, mouse and rat. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
OSA00011G
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
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Sheep antibody to ATG3
