|
|
Code
OSM00020W
$240USD
ID Tag
Sh11-281007-WS
Immunogen
A synthetic peptide from human MAP1LC3C conjugated to an immunogenic carrier protein has been used as the antigen.
Accession
Also known
Microtubule-associated proteins 1A/1B light chain 3C, MAP1LC3C, MAP1A/MAP1B LC3 C, MAP1A/1B light chain 3 C, MAP1 light chain 3-like protein 3, Autophagy-related protein LC3 C, Autophagy-related ubiquitin-like modifier LC3C
Target
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed.
FUNCTION: Probably involved in formation of autophagosomal vacuoles (autophagosomes).
SUBUNIT: 3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins.
SUBCELLULAR LOCATION: Cytoplasm. Intracytoplasmic membrane; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note: LC3-II binds to the autophagic membranes.
TISSUE SPECIFICITY: Most abundant in placenta, lung and ovary.
The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
FUNCTION: Probably involved in formation of autophagosomal vacuoles (autophagosomes).
SUBUNIT: 3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins.
SUBCELLULAR LOCATION: Cytoplasm. Intracytoplasmic membrane; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note: LC3-II binds to the autophagic membranes.
TISSUE SPECIFICITY: Most abundant in placenta, lung and ovary.
The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Gregory S.G, et al. Nature 441 (7091), 315-321(2006).
2. Tanida I, et al. J. Biol. Chem. 279 (35), 36268-36276(2004).
3. He H, et al. J. Biol. Chem. 278 (31), 29278-29287(2003).
2. Tanida I, et al. J. Biol. Chem. 279 (35), 36268-36276(2004).
3. He H, et al. J. Biol. Chem. 278 (31), 29278-29287(2003).
Limitation
For research use only
Related
products
products
|
Code
OSM00020W
$240USD
Unit size
150 µl
Conjugate
Unconjugated antibody
Host
Sheep
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB. Use at a dilution of 1 : 200 to 1 : 2000. The optimal dilution should be determined by the end user.
Specificity
Specific for MAP1LC3C.
Spcs X-react.
Human. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 150 µl of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
OSM00020W
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
⚬ Products
Sheep antibody to MAP1 LC3C
