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Code
OSR00101W
$240USD    Buy Now 

ID Tag
Rb1485-120610-WS

Immunogen
A synthetic peptide from aa region 110-160 of human ASIC4 conjugated to blue carrier protein was used as the antigen. The antigen is homologous in rat and mouse.

Accession

Also known
Amiloride sensitive cation channel 4 pituitary, acid-sensing ion channel 4, spinal chord ASIC, Spasic, BNAC4, MGC17248, MGC24860

Target
FUNCTION: Probable cation channel with high affinity for sodium. These channels have been implicated in synaptic transmission, pain perception as well as mechanoperception.
SUBUNIT: Homotetramer or heterotetramer with other ASIC proteins (Probable).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: This gene is predominantly expressed in the pituitary gland. Expressed in brain, spinal chord and dorsal root ganglion (DRG). Expressed by a subset of sensory neurons in the DRG. Expressed by granule cells in the cerebellar cortex. In hippocampus, expression is detected in dentate gyrus granule cells, in pyramidal cells of CA1-CA3 subfields and in interneurons of the striatum oriens and radiatum of all subfields. In cerebral cortex expressed in small, medium and large pyramidal cells in layers 2, 3 and 5 respectively. Also expressed in striatum, globus pallidus, inferior and superior calliculi, amygdala, magnocellular preoptic nucleus, islands of Calleja and large neurons of olfactory tubercules.
DEVELOPMENTAL STAGE: Highly expressed in newborn spinal chord but hardly detected in the cerebellum compared to adult. Expressed at postnatal day 1 in ependymal cells lining the central canal of spinal chord and in motor neurons. In adult, expression decreases in ependymal cells and increases in motor neurons. The number of positive interneurons decreases but the individual interneuron expression increases in adult spinal chord compared to newborn.
MISCELLANEOUS: In vitro, has no proton-gated channel activity.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Gruender S, et al. NeuroReport 11:1607-1611(2000).

Limitation
For research use only

Code
OSR00101W
$240USD    Buy Now 

Unit size
100 ul

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for ASIC4.

Spcs X-react.
Human, rat and mouse. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material.

Code
GSA00001P
$120USD   

ID Tag
92893-111

Unit size
50 µg

Accession

Also known
Amiloride sensitive cation channel 4 pituitary, acid-sensing ion channel 4, spinal chord ASIC, Spasic, BNAC4, MGC17248, MGC24860

Target
This is the immunising peptide for the product Rabbit antibody to internal region of amiloride sensitive cation channel 4 (ACCN4, ASIC4): whole serum (OSR00101W)

Purity
>83%

Applications
Control peptide

Format
Lyophilised

Reconstitution
This peptide is soluble in water.

Storage
Maintain refrigerated at 2-8°C for up to 12 months. After reconstitution keep at -20°C. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Gruender S, et al. NeuroReport 11:1607-1611(2000).
2. Akopian A.N, et al. NeuroReport 11:2217-2222(2000).

Limitation
For research use only

Related Products
OSR00101W

⚬ Products
Rabbit antibody to ASIC4 (110-160)
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

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