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Code
OSC00329W
$240USD
ID Tag
Gp421-140620-WS
Immunogen
Synthetic peptide "ASYQTQTNSPRRARSVA" aa 671-688 of SARS spike glycoprotein polybasic region conjugated to blue carrier protein was used as the antigen.
Accession
Also known
SARS-Cov-2, Surface glycoprotein
Target
Function: Spike protein S1:attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein. The stalk domain of S contains three hinges, giving the head unexpected orientational freedom. Uses human TMPRSS2 for priming in human lung cells which is an essential step for viral entry. Can be alternatively processed by host furin. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.
Spike protein S2: mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.
Spike protein S2': Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.
Spike glycoprotein: May down-regulate host tetherin (BST2) by lysosomal degradation, thereby counteracting its antiviral activity.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Hoffmann M. ertal. Cell 181:1-10(2020)
Limitation
For research use only
Code
OSC00329W
$240USD
Unit size
100 ul
Conjugate
Unconjugated antibody
Host
Guinea pig
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB. A dilution of 1: 1000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for SARS-Cov-2 spike glycoprotein polybasic region
Spcs X-react.
SARS-CoV-2
Format
Lyophilised
Reconstitution
Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
OSC00329W
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
⚬ Products
Guinea pig antibody to SARS-Cov-2 spike glycoprotein-polybasic region (671-688)
