A synthetic peptide from aa region 350-400 of mouse SLC6A19 conjugated to blue carrier protein was used as the antigen. The antigen is homologous in rat.
Sodium-dependent neutral amino acid transporter B(0)AT1, Solute carrier family 6 member 19, SLC6A19, System B(0) neutral amino acid transporter antibody,
Function: Transporter that mediates resorption of neutral amino acids across the apical membrane of renal and intestinal epithelial cells. This uptake is sodium-dependent and chloride-independent. Requires CLTRN in kidney or ACE2 in intestine for cell surface expression and amino acid transporter activity.
Subcellular Location: Cell membrane; Multi-pass membrane protein.
Note: Localizes in small intestine brush border membranes (at protein level).
Expression: Predominantly expressed in kidney and small intestine. Expressed in the intestinal brush border (at protein level). Expression not observed in other organs, such as lung, skeletal muscle, brain, liver and pancreas. In kidney, expression is localized in the renal cortex but not in the medulla. Substantial amounts of expression in the proximal tubules. The distal nephron segments and the glomeruli are consistently negative. In the small intestine, expression is exclusively localized in villus enterocytes. High resolution of the hybridization-positive villi reveals a gradient of expression with the highest levels in apical cells. Not detected in crypt cells or in any other cell types of the small intestine.
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
12 months after reconstitution
This item will be shipped to you at ambient temperature in a lyophilised form.
1. Broeer A., et al. J. Biol. Chem. 279:24467-24476(2004)
For research use only
NZ white rabbit
Polyclonal, whole serum
IHC, WB. A dilution of 1: 1000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specific for SLC6A19.
Mouse, rat, marmoset. Other species not yet tested.
Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material.