|
|
Code
OSA00261W
$240USD
ID Tag
Rb2260-271213-WS
Immunogen
A synthetic peptide from aa region 200-250 of human Aggrecan conjugated to blue carrier protein was used as the antigen. The peptide is homologous with rat and mouse sequences.
Accession
Also known
Cartilage specific proteoglycan core protein, CSPCP, Chondroitin sulfate proteoglycan core protein 1, Chondroitin sulfate proteoglycan 1, Aggrecan core protein 2
Target
FUNCTION: This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.
SUBCELLULAR LOCATION: Secreted ⺠extracellular space ⺠extracellular matrix
Tissue specificity: Restricted to cartilages
SUBCELLULAR LOCATION: Secreted ⺠extracellular space ⺠extracellular matrix
Tissue specificity: Restricted to cartilages
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Morawski M1, et al. Int J Biochem Cell Biol. 2012 May;44(5):690-3
Limitation
For research use only
Related
products
products
|
Code
OSA00261W
$240USD
Unit size
100 µl
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for Aggrecan.
Spcs X-react.
Human. Other species not yet tested but expected to work in rat and mouse.
Format
Lyophilised
Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
OSA00261W
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OSA00261W
$240USD
|
|
IHC on paraffin sections of mouse cerebellum tissue using Rabbit antibody to Aggrecan (200-250): OSA00261W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin. |
|
|
IHC on paraffin sections of mouse cerebellum tissue using Rabbit antibody to Aggrecan (200-250): OSA00261W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin. |
|
|
IHC on paraffin sections of mouse cerebellum tissue using Rabbit antibody to Aggrecan (200-250): OSA00261W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin. |
|
|
IHC on paraffin sections of mouse cerebellum tissue using Rabbit antibody to Aggrecan (200-250): OSA00261W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin. |
|
|
IHC on paraffin sections of mouse cerebellum tissue using Rabbit antibody to Aggrecan (200-250): OSA00261W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin. |
|
|
IHC on paraffin sections of mouse cerebellum tissue using Rabbit antibody to Aggrecan (200-250): OSA00261W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin. |
|
|
IHC on paraffin sections of mouse spinal cord tissue using Rabbit antibody to Aggrecan (200-250): OSA00261W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin. |
|
|
IHC on paraffin sections of mouse spinal cord tissue using Rabbit antibody to Aggrecan (200-250): OSA00261W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin. |
|
|
IHC on paraffin sections of mouse spinal cord tissue using Rabbit antibody to Aggrecan (200-250): OSA00261W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin. |
|
|
IHC on paraffin sections of mouse spinal cord tissue using Rabbit antibody to Aggrecan (200-250): OSA00261W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin. |
|
|
IHC on paraffin sections of mouse spinal cord tissue using Rabbit antibody to Aggrecan (200-250): OSA00261W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin. |
|
|
IHC on paraffin sections of mouse spinal cord tissue using Rabbit antibody to Aggrecan (200-250): OSA00261W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin. |
|
|
IHC on paraffin sections of mouse spinal cord tissue using Rabbit antibody to Aggrecan (200-250): OSA00261W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin. |
⚬ Products
Rabbit antibody to Aggrecan (200-250)
