A synthetic peptide from aa region 1310-1360 of mouse MAGI3 conjugated to an immunogenic carrier protein was used as the antigen.
Membrane associated guanylate kinase, WW and PDZ domain containing protein 3, Membrane associated guanylate kinase inverted 3, MAGI-3
FUNCTION: Acts as a scaffolding protein at cell-cell junctions, thereby regulating various cellular and signaling processes. Cooperates with PTEN to modulate the kinase activity of AKT1. Its interaction with PTPRB and tyrosine phosphorylated proteins suggests that it may link receptor tyrosine phosphatase with its substrates at the plasma membrane. In polarized epithelial cells, involved in efficient trafficking of TGFA to the cell surface. Regulates the ability of LPAR2 to activate ERK and RhoA pathways. Regulates the JNK signaling cascade via its interaction with FZD4 and VANGL2.
Subcellular location: Cell membrane; Peripheral membrane protein. Cell junction âº tight junction. Nucleus. Note: Concentrates in specific sites at the plasma membrane and in the nucleus. In epithelial cells, it localizes at tight junctions.
Tissue specificity: Widely expressed. Colocalizes with TGFA in neurons in the cortex and dentate gyrus, as well as in ependymal cells and some astrocytes (at protein level). Present in lens epithelium.
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
12 months after reconstitution
This item will be shipped to you at ambient temperature in a lyophilised form.
1. Franklin J.L, et al. Exp. Cell Res. 303:457-470(2005)
For research use only
NZ white rabbit
Polyclonal, whole serum
IHC, WB (confirmed by recombinant protein). A dilution of 1: 200 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specific for MAGI3.
Mouse. Other species not yet tested.
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.