⚬ Services
Code
OSR00377W
$240USD    Buy Now 

ID Tag
Rb2143-011013-WS

Immunogen
A synthetic peptide from aa region 750-800 of mouse Rubicon conjugated to an immunogenic carrier protein has been used as the antigen. The peptide is homologous in human and rat.

Accession

Also known
Run domain Beclin 1 interacting and cysteine rich containing protein, Baron

Target
Function: Negatively regulates endocytic trafficking. Impairs the autophagosome maturation process. May inhibit PIK3C3 activity.
Subcellular location: Late endosome. Lysosome. Early endosome. Note: Predominantly located in late endosomes/lysosomes, only partially detected in early endososome and not at all in the Golgi apparatus.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Matsunaga K, et al. Nat. Cell Biol. 11:385-396(2009)

Limitation
For research use only

Code
OSR00377W
$240USD    Buy Now 

Unit size
100 µl

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. Use at a dilution of 1 : 200 to 1 : 2000. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for Rubicon.

Spcs X-react.
Mouse, human, rat. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

⚬ Products
Rabbit antibody to Rubicon (750-800)
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

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