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Code
OSP00184W
$240USD    Buy Now 

ID Tag
Rb2133-230713-WS

Immunogen
A synthetic peptide from aa region 1050-1100 of human Perilipin 4 conjugated to an immunogenic carrier protein was used as the antigen.

Accession

Also known
Adipocyte protein S3-12, PLIN4

Target
Function: May play a role in triacylglycerol packaging into adipocytes. May function as a coat protein involved in the biogenesis of lipid droplets.
Subcellular location: Cell membrane. Cytoplasm. Lipid droplet. Note: Nascent lipid droplet surface-associated; association with lipid droplets is triacylglycerol synthesis-dependent

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Dalen K.T, et al. Diabetes 53:1243-1252(2004)

Limitation
For research use only

Code
OSP00184W
$240USD    Buy Now 

Unit size
100 µl

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB (confirmed by recombinant protein). A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for Perilipin 4.

Spcs X-react.
Human. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSP00184W
$240USD    Buy Now 


Rabbit antibody to Perilipin 4 (1050-1100) IHC on paraffin sections of human large intestine tissue using Rabbit antibody to Perilipin 4 (1050-1100): OSP00184W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to Perilipin 4 (1050-1100) IHC on paraffin sections of human uterus tissue using Rabbit antibody to Perilipin 4 (1050-1100): OSP00184W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to Perilipin 4 (1050-1100) IHC on paraffin sections of human appendix tissue using Rabbit antibody to Perilipin 4 (1050-1100): OSP00184W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to Perilipin 4 (1050-1100) IHC on paraffin sections of human small intestine tissue using Rabbit antibody to Perilipin 4 (1050-1100): OSP00184W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to Perilipin 4 (1050-1100) IHC on paraffin sections of human prostate tissue using Rabbit antibody to Perilipin 4 (1050-1100): OSP00184W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to Perilipin 4 (1050-1100) IHC on paraffin sections of human breast cancer tissue using Rabbit antibody to Perilipin 4 (1050-1100): OSP00184W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to Perilipin 4 (1050-1100) IHC on paraffin sections of human large intestine tissue using Rabbit antibody to Perilipin 4 (1050-1100): OSP00184W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer. Sections were counterstained with Harris Hematoxylin.
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Rabbit antibody to Perilipin 4 (1050-1100)
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

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