⚬ Services
Code
OSA00200W
$240USD    Buy Now 

ID Tag
Rb2577-230615-WS

Immunogen
A synthetic peptide from aa region 10-50 of rat MT-ATP6 conjugated to blue carrier protein was used as the antigen.

Accession

Also known
F-ATPase protein 6, ATP6, ATPASE6

Target
FUNCTION: Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Key component of the proton channel; it may play a direct role in the translocation of protons across the membrane.
Subcellular location: Mitochondrion inner membrane; Multi-pass membrane protein

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1.Speir J.A, et al. Immunity 14:81-92(2001)

Limitation
For research use only

Code
OSA00200W
$240USD    Buy Now 

Unit size
100 µl

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1: 1000 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for ATP6.

Spcs X-react.
Rat. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSA00200W
$240USD    Buy Now 


Rabbit antibody to MT-ATP6 (100-150) Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1 : 2000 incubated at 4C overnight. Click on eLAB BOOK tab to see the details.

⚬ Products
Rabbit antibody to MT-ATP6 (100-150)
Content

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APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
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