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Code
OSP00172W
$240USD    Buy Now 

ID Tag
Rb1887-141012-WS

Immunogen
A synthetic peptide from aa region 330-380 of human PICALM conjugated to an immunogenic carrier protein was used as the antigen.

Accession

Also known
Phosphatidylinositol-binding clathrin assembly protein, Clathrin assembly lymphoid myeloid leukemia protein, CALM, FIT1

Target
FUNCTION: Assembly protein recruiting clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction.
Note=A chromosomal aberration involving PICALM is found in diffuse histiocytic lymphomas.
SUBCELLULAR LOCATION: Membrane › clathrin-coated pit. Golgi apparatus. Cytoplasmic vesicle › clathrin-coated vesicle. Note: Colocalized with clathrin in the Golgi area.
TISSUE SPECIFICITY: Expressed in all tissues examined.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Silliman C.C, et al. Leukemia 12:1404-1410(1998)
2. Tebar F, et al. Mol. Biol. Cell 10:2687-2702(1999)

Limitation
For research use only

Code
OSP00172W
$240USD    Buy Now 

Unit size
100 µl

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not tested in other applications.

Specificity
Specific for PICALM.

Spcs X-react.
Human, rat, mouse. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

⚬ Products
Rabbit antibody to PICALM
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

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