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Code
OSA00164W
$240USD
ID Tag
Rb1729-020111-WS
Immunogen
A synthetic peptide from c-terminal region of human ATP8B1 conjugated to blue carrier protein was used as the antigen.
Accession
Also known
Probable phospholipid-transporting ATPase IC, ATPase class I type 8B member 1, Familial intrahepatic cholestasis type 1, ATPIC, FIC1, PFIC
Target
FUNCTION: Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules. May play a role in asymmetric distribution of phospholipids in the canicular membrane. May have a role in transport of bile acids into the canaliculus, uptake of bile acids from intestinal contents into intestinal mucosa or both. In cooperation with ABCB4 may be involved in establishing integrity of the canalicular membrane thus protecting hepatocytes from bile salts. Together with TMEM30A is involved in uptake of the synthetic drug alkylphospholipid perifosine. Involved in the microvillus formation in polarized epithelial cells; the function seems to be independent from its flippase activity. Required for the preservation of cochlear hair cells in the inner ear. May act as cardiolipin transporter during inflammatory injury.
Subcellular location: Membrane; Multi-pass membrane protein
Tissue specificity: Found in most tissues except brain and skeletal muscle. Most abundant in pancreas and small intestine.
Subcellular location: Membrane; Multi-pass membrane protein
Tissue specificity: Found in most tissues except brain and skeletal muscle. Most abundant in pancreas and small intestine.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Bull L.N, et al. Nat. Genet. 18:219-223(1998)
Limitation
For research use only
Code
OSA00164W
$240USD
Unit size
100 ul
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB (confirmed by recombinant protein). A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for ATP8B1.
Spcs X-react.
Human, mouse, rat. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
OSA00164W
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OSA00164W
$240USD
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IHC on paraffin sections of human bowel tissue using Rabbit antibody to ATP8B1: OSA00164W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of human muscle tissue (negative control) using Rabbit antibody to ATP8B1: OSA00164W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of human bowel tissue using Rabbit antibody to ATP8B1: OSA00164W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of human bowel tissue using Rabbit antibody to ATP8B1: OSA00164W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of human bowel tissue using Rabbit antibody to ATP8B1: OSA00164W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin. |
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