Rb1694-021210-WS

A synthetic peptide from n-terminal region of human PITX3 conjugated to an immunogenic carrier protein was used as the antigen.

PITX2_HUMAN
PITX2_MOUSE
PITX2_RAT

Pituitary homeobox 3, Homeobox protein PITX3, Paired-like homeodomain transcription factor 3, PTX3

FUNCTION: Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. In addition to its importance during development, it also has roles in the long-term survival and maintenance of the mdDA neurons. Activates NR4A2/NURR1-mediated transcription of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons. Acts by decreasing the interaction of NR4A2/NURR1 with the corepressor NCOR2/SMRT which acts through histone deacetylases (HDACs) to keep promoters of NR4A2/NURR1 target genes in a repressed deacetylated state. Essential for the normal lens development and differentiation. Plays a critical role in the maintenance of mitotic activity of lens epithelial cells, fiber cell differentiation and in the control of the temporal and spatial activation of fiber cell-specific crystallins. Positively regulates FOXE3 expression and negatively regulates PROX1 in the anterior lens epithelium, preventing activation of CDKN1B/P27Kip1 and CDKN1C/P57Kip2 and thus maintains lens epithelial cells in cell cycle.
Defects in PITX3 are a cause of cataract autosomal dominant (ADC).
Defects in PITX3 are a cause of anterior segment mesenchymal dysgenesis (ASMD).
Defects in PITX3 are the cause of cataract posterior polar type 4 (CTPP4).
SUBCELLULAR LOCATION: Nucleus
TISSUE SPECIFICITY: Highly expressed in developing eye lens.

Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

12 months after reconstitution

This item will be shipped to you at ambient temperature in a lyophilised form.

1. Semina E.V, et al. Nat. Genet. 19:167-170(1998)
2. Berry V, et al. J. Med. Genet. 41:E109-E109(2004)
3. Gauci S, et al. Anal. Chem. 81:4493-4501(2009)

For research use only

100 µl

Unconjugated antibody

NZ white rabbit

Whole serum

Polyclonal

Polyclonal, whole serum

IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not tested in other applications.

Specific for PITX2.

Human, mouse, rat. Other species not yet tested.

Lyophilised

Reconstitute in 100 µl of MQ water. Centrifuge to remove any insoluble material.

Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

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