Rb1686-021210-WS

A synthetic peptide from the n-terminal region of human CLCKB conjugated to an immunogenic carrier protein was used as the antigen.

CLCKB_HUMAN
CLCKA_HUMAN

Chloride channel Kb, ClC-K2, CLCKB; CLCNKA, Chloride channel protein ClC-Ka, Chloride channel Ka, ClC-K1

FUNCTION: Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume; membrane potential stabilization, signal transduction and transepithelial transport. May be important in urinary concentrating mechanisms.
Subcellular location: Cell membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Note: Recruited to the plasma membrane in the presence of BSND protein, whereas remains in the Golgi in its absence.
Tissue specificity: Expressed predominantly in the kidney. Expressed in all segments of the nephron examined, including the S2 segment and the glomerulus.
Defects in CLCNKA are a cause of Bartter syndrome type 4B (BS4B). A digenic, recessive disorder characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria. Bartter syndrome type 4B is associated with sensorineural deafness.

Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

12 months after reconstitution

This item will be shipped to you at ambient temperature in a lyophilised form.

1. Kieferle S, et al. Proc. Natl. Acad. Sci. U.S.A. 91:6943-6947(1994)
2. Hayama A, et al. Histochem. Cell Biol. 119:485-493(2003)

For research use only

100 µl

Unconjugated antibody

NZ white rabbit

Whole serum

Polyclonal

Polyclonal, whole serum

IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specific for CLCKB/A.

Human. Other species not yet tested but expected to work in mouse and rat.

Lyophilised

Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

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- Click to download the Material Safety Data Sheet