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Code
OSN00067W
$240USD    Buy Now 

ID Tag
Rb1701-021210-WS

Immunogen
A synthetic peptide from aa region 980-1030 of human NMDAR3A conjugated to blue carrier protein was used as the antigen.

Accession

Also known
NMD3A, Glutamate [NMDA] receptor subunit 3A, Glutamate receptor chi-1, N-methyl-D-aspartate receptor, N-methyl-D-aspartate receptor subtype 3A, NR3A, NMDAR-L, NMDAR-L1

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Andersson O, et al. Genomics 78:178-184(2001)

Limitation
For research use only

Code
OSN00067W
$240USD    Buy Now 

Unit size
100 ul

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for NMDAR3A.

Spcs X-react.
Human. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 ul of MQ water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSN00067W
$240USD    Buy Now 


Rabbit antibody to NMDAR3A (980-1030) IHC on paraffin sections of human brain tissue using Rabbit antibody to NMDAR3A (980-1030): OSN00067W.

 

HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module.

Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm.

Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions.

Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer).

Sections were counterstained with Harris Hematoxylin.

Rabbit antibody to NMDAR3A (980-1030) IHC on paraffin sections of human brain tissue using Rabbit antibody to NMDAR3A (980-1030): OSN00067W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to NMDAR3A (980-1030) IHC on paraffin sections of human brain tissue using Rabbit antibody to NMDAR3A (980-1030): OSN00067W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to NMDAR3A (980-1030) IHC on paraffin sections of human brain tissue using Rabbit antibody to NMDAR3A (980-1030): OSN00067W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.
⚬ Products
Rabbit antibody to NMDAR3A (980-1030)
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

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