Melanopsin antibody, OPSIN4 antibody, OPSIN 4 antibody

OVERVIEW
SPECS
CONTROL PEPTIDE
MSDS/DS

Code

OSM00085W

$240USD

ID Tag

Rb788-140209-WS

Immunogen

A synthetic peptide from c-terminal region of human Melanopsin (OPN4, Opsin-4, MOP) conjugated to blue carrier protein was used as the antigen.

Accession

OPN4_HUMAN

Also known

Melanopsin, opsin-4, OPN4, MOP, OPN 4, MGC142118

Target

Opsins are members of the guanine nucleotide-binding protein (G protein)-coupled receptor superfamily. This gene encodes a photoreceptive opsin protein that is expressed within the ganglion and amacrine cell layers of the retina. In mouse, retinal ganglion cell axons expressing this gene projected to the suprachiasmatic nucleus and other brain nuclei involved in circadian photoentrainment. In mouse, this protein is coupled to a transient receptor potential (TRP) ion channel through a G protein signaling pathway and produces a physiologic light response via membrane depolarization and increased intracellular calcium. The protein functions as a sensory photopigment and may also have photoisomerase activity. Experiments with knockout mice indicate that this gene attenuates, but does not abolish, photoentrainment. Alternative splicing results in multiple transcript variants encoding different isoforms.
FUNCTION: Photoreceptor required for regulation of circadian rhythm. Contributes to pupillar reflex and other non-image forming responses to light. May be able to isomerize covalently bound all-trans retinal back to 11-cis retinal.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. Note: Found in soma, dendrites and proximal part of axons of certain retinal ganglion cells .
TISSUE SPECIFICITY: Eye. Expression is restricted within the ganglion and amacrine cell layers of the retina.

Storage

Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date

12 months after reconstitution

Shipping

This item will be shipped to you at ambient temperature in a lyophilised form.

References

1. Provencio I, et al. J. Neurosci. 20:600-605(2000).

Limitation

For research use only

Code

OSM00085W

$240USD

Unit size

100 ul

Conjugate

Unconjugated antibody

Host

NZ white rabbit

Purity

Whole serum

Clonality

Polyclonal

Isotype

Polyclonal, whole serum

Applications

IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity

Specific for opsin-4.

Spcs X-react.

Human. Other species not yet tested.

Format

Lyophilised

Reconstitution

Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material.

Note

Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code

OSM00085W