A synthetic peptide from aa region 190-250 of human SRD5A2 conjugated to blue carrier protein was used as the antigen.
S5A2, 3-oxo-5-alpha-steroid 4-dehydrogenase 2, Steroid 5-alpha-reductase 2, S5AR 2, 5 alpha-SR2, Type II 5-alpha reductase, SR type 2
Function: Converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology. Defects in SRD5A2 are the cause of pseudovaginal perineoscrotal hypospadias (PPSH)
; also known as male pseudohermaphroditism due to 5-alpha-reductase deficiency or 5-ARD deficiency. PPSH is a rare form of male pseudohermaphroditism in which affected males develop normal internal urogenital tracts but fail to develop external male structures. Individuals with PPSH have testicles and tend to have a vagina and labia, but with a small penis capable of ejaculation instead of a clitoris (this penis, however, appears to be a clitoris at birth). These individuals are normally raised as girls. However at puberty their testes will descend, their voice will deepen and they often will develop a male sexual identity. They develop only limited facial hair and will not experience male-pattern baldness. Jeffrey Eugenides won a Pulitzer prize for his 2003 novel 'Middlesex' which describes the life an individual with such a deficiency.
Subcellular location: Microsome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein
Tissue specificity: Expressed in high levels in the prostate and many other androgen-sensitive tissues.
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
12 months after reconstitution
This item will be shipped to you at ambient temperature in a lyophilised form.
1. Andersson S, et al. Nature 354:159-161(1991)
For research use only
NZ white rabbit
Polyclonal, whole serum
IHC, WB. A dilution of 1: 4000 is recommended for WB and 1: 2000 for IHC-P. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specific for SRD5A2.
Human. Other species not yet tested but expected to work in rat and mouse.
Lyophilised. This product contains 0.02% benzalkonium chloride.
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.