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Code
OSR00358W
$240USD
ID Tag
Rb1285-140210-WS
Immunogen
A synthetic peptide from c-terminal region of mature human Repulsive guidance molecule C (RGMC, Hemojuvelin) conjugated to an immunogenic carrier protein was used as the antigen.
Accession
Also known
RGMC, Hemojuvelin, Hemochromatosis type 2 protein, RGM domain family member C, HFE2, HJV
Target
Function: Member of the repulsive guidance molecule (RGM) family. Involved in iron metabolism. Acts as a bone morphogenetic protein (BMP) coreceptor. Enhancement of BMP signaling regulates hepcidin (HAMP) expression and iron metabolism. May cooperate with hepcidin to restrict iron absorption in the gut. Could represent the cellular receptor for hepcidin.
Subcellular location: Cell membrane; Lipid-anchor ⺠GPI-anchor
Tissue specificity: Adult and fetal liver, heart, and skeletal muscle.
Subcellular location: Cell membrane; Lipid-anchor ⺠GPI-anchor
Tissue specificity: Adult and fetal liver, heart, and skeletal muscle.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Papanikolaou G, et al. Nat. Genet. 36:77-82(2004)
2. Ota T, et al. Nat. Genet. 36:40-45(2004)
3. Babitt J.L, et al. Nat. Genet. 38:531-539(2006)
2. Ota T, et al. Nat. Genet. 36:40-45(2004)
3. Babitt J.L, et al. Nat. Genet. 38:531-539(2006)
Limitation
For research use only
Code
OSR00358W
$240USD
Unit size
100 µl
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not tested in other applications.
Specificity
Specific for RGMC.
Spcs X-react.
Human, rat, mouse . Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
OSR00358W
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OSR00358W
$240USD
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IHC-P on paraffin sections of rat lung. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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IHC-P on paraffin sections of rat lung. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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