⚬ Services
Code
OSD00032W
$240USD    Buy Now 

ID Tag
Rb1288-140210-WS

Immunogen
A synthetic peptide from the internal region of human Dorsal repulsive axon guidance protein (Draxin, Neucrin) conjugated to an immunogenic carrier protein was used as the antigen.

Accession

Also known
DRAXI, Draxin, Neucrin, C1orf187

Target
FUNCTION: Chemorepulsive axon guidance protein required for the development of spinal cord and forebrain commissures. Acts as a chemorepulsive guidance protein for commissural axons during development. Able to inhibit or repel neurite outgrowth from dorsal spinal cord. Inhibits the stabilization of cytosolic beta-catenin (CTNNB1) via its interaction with LRP6, thereby acting as an antagonist of Wnt signaling pathway
SUBCELLULAR LOCATION: Secreted
TISSUE SPECIFICITY: Predominantly expressed in developing neural tissues. Expressed in many brain regions, including the olfactory bulb, cortex, midbrain, cerebellum and pontine nuclei in postnatal day 0 (P0). Detected in the dorsal spinal cord and commissural axons. In the forebrain commissures, it is expressed in the regions that surround the corpus callosum, hippocampal commissure, and anterior commissure, such as the midline glial cells, indusium griseum glia, and glial wedge.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Clark H.F, et al. Genome Res. 13:2265-2270(2003)
2. Gregory S.G, et al. Nature 441:315-321(2006)
3. Zhang Z, et al. Protein Sci. 13:2819-2824(2004)

Limitation
For research use only

Code
OSD00032W
$240USD    Buy Now 

Unit size
100 µl

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not tested in other applications.

Specificity
Specific for Draxin.

Spcs X-react.
Human. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

⚬ Products
Rabbit antibody to Neucrin
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

Watch Videos

Autoperfuserâ„¢ OS2100
Red Carpet: A Histology Carpet for Slides