A synthetic peptide from aa region 40-90 of human SOD1 conjugated to blue carrier protein was used as the antigen. The antigen is homologous in rat and mouse.
SOD1, Superoxide dismutase 1, SODC
Function: Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
Catalytic activity: 2 superoxide + 2 H(+) = O(2) + H(2)O(2).
Subcellular location: Cytoplasm.
Involvement in disease: Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1). ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
The protein (both wild-type and ALS1 variants) has a tendency to form fibrillar aggregates in the absence of the intramolecular disulfide bond or of bound zinc ions. These aggregates may have cytotoxic effects. Zinc binding promotes dimerization and stabilizes the native form.
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
12 months after reconstitution
This item will be shipped to you at ambient temperature in a lyophilised form.
1. Sherman L, et al. Proc. Natl. Acad. Sci. U.S.A. 80:5465-5469(1983)
2. Ota T, et al. Nat. Genet. 36:40-45(2004)
For research use only
NZ white rabbit
Polyclonal, whole serum
IHC, WB. A dilution of 1: 3000 is recommended for WB and to 1: 1000 fro IHC-P. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specific for SOD1.
Human. Other species not yet tested but expected to work in rat, mouse and guinea pig.
Reconstitute in 150 µl of sterile water. Centrifuge to remove any insoluble material.