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Code
OSS00205W
$260USD    Buy Now 

ID Tag
Rb1160-260909-WS

Immunogen
A synthetic peptide from human 5HT7 R conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat, mouse and guinea pig.

Accession

Also known
5-hydroxytryptamine receptor 7, 5-HT-7, Serotonin receptor 7, HTR7

Target
FUNCTION: This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase.
Subcellular location: Cell membrane; Multi-pass membrane protein.
Tissue specificity: Isoform A is the predominant isoform in spleen, caudate and hippocampus. Isoform B is expressed at lower levels. Isoform D is a minor isoform in term of expression.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Heidmann D.E.A, et al. J. Neurochem. 68:1372-1381(1997)

Limitation
For research use only

Code
OSS00205W
$260USD    Buy Now 

Unit size
100 ul

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1: 1000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for 5HT7R receptor.

Spcs X-react.
Human, rat, guinea pig, mouse. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material.

Code
OSS00205P
$120USD   

ID Tag
74234-29

Unit size
50 µg

Accession

Also known
5-hydroxytryptamine receptor 7, 5-HT-7, Serotonin receptor 7, HTR7

Target
This is the immunising peptide for the product Rabbit antibody to 5HT7 R (300-350) (OSS00205W).

Purity
>80%

Applications
Control peptide

Format
Lyophilised

Reconstitution
This peptide is soluble in PBS and water.

Storage
Maintain refrigerated at 2-8°C for up to 12 months. After reconstitution keep at -20°C. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

Limitation
For research use only

Related Products
OSS00205W

Code
OSS00205W
$260USD    Buy Now 


Rabbit antibody to 5HT7 R IHC-P on paraffin sections of rat brain.
The animal was perfused using Autoperfuser at a pressure of 130 mmHg  with 300 ml 4% FA being processed for paraffin embedding. HIER:  Tris-EDTA, pH 9 for 20 min using Thermo PT Module.
Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm.
Detection was done using Novolink HRP polymer from Leica following  manufacturers instructions; DAB chromogen: Candela DAB chromogen from  Osenses.
Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.
Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to 5HT7 R IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
⚬ Products
Rabbit antibody to 5HT7 R
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

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